Abstract
The patterns of quinolizidine alkaloids in cell cultures of 10 species of Fabaceae
were analyzed by high-resolution GLC and GLC-MS and compared with the alkaloids present
in the leaves of the respective plants. Lupanine was produced in all 10 cell suspension
cultures as the main alkaloid. It was accompanied by sparteine, tetrahydrorhombifoline,
17-oxosparteine, 13-hydroxylupanine, 4-hydroxylupanine, 17-oxolupanine, and 13-hydroxylupanine
esters as minor alkaloids in some species. The alkaloid patterns of the plants differed
markedly in that α-pyridone alkaloids were the major alkaloids in the genera Cytisus,
Genista, Laburnum and Sophora but were not accumulated in the cell cultures. These
data further support the assumption that the pathway leading to lupanine is the basic
pathway of quinolizidine alkaloids biosynthesis and that the other alkaloids are derived
from lupanine.
Key Word Index
Lupinus - Cytisus - Laburnum - Baptisia - Genista - Sophora - Fabaceae - Quinolizidine
Alkaloids - Cell Suspension Cultures - Capillary GLC-MS
